Kinesin Family, Member 20B (KIF20B)

The transition from G2 to M phase is induced by M phase-promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M-phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M-phase enzymatic activities and structures. Sequence analysis predicted that the partial MPHOSPH1 cDNA encodes a 566-amino acid protein, the first 302 amino acids of which form a coiled-coil alpha helix. The authors determined that the MPM2 antibody-reactive sites of the MPHOSPH proteins consist of 5-amino acid stretches containing a hydrophobic residue, the putative phosphorylated residue (proline), another hydrophobic residue, and finally a basic or hydrophobic residue.