LEM Domain Containing Protein 1 (LEMD1)

Northern blot analysis detected a 0.7-kb LEMD1 transcript expressed in testis only. RT-PCR revealed 6 alternatively spliced LEMD1 transcripts in testis.The longest deduced protein, LEMD1A, contains 181 amino acids and has an N-terminal LEM domain. Of the 6 LEMD1 variants, LEMD1B, which encodes a deduced 29-amino acid protein lacking the LEM domain, showed highest expression in testis. LEMD1F, which encodes a 67-amino acid protein containing the N-terminal LEM domain, was detected in 17 of 18 colon cancer tissues examined. Epitope-tagged LEMD1F was expressed in transfected COS-7 cells at the nuclear membrane and colocalized with transfected BAF (BANF1).