Leucine Rich Repeat Containing Protein 1 (LRRC1)

LRRC1 and a C-terminal LRRC1 peptide interacted with the PDZ domains of DLG1 and PSD95 (DLG4). The deduced protein contains 16 leucine-rich repeats and a LAP-specific domain, but no PDZ domain. LRRC1 shares 60%, 42%, and 40% amino acid identity with scribble, rat densin-180, and erbin, respectively. Northern blot analysis detected a 3.5-kb transcript expressed predominantly in placenta, kidney, pancreas, prostate, testis, colon, thyroid, and adrenergic glands. Western blot analysis detected endogenous LRRC1 at an apparent molecular mass of about 62 kD in a human colon epithelial (Caco2) cell line. Immunolocalization and confocal sections of permeabilized Caco2 cells detected LRRC1 expression on the basolateral surface.