Leucine Rich Repeat Containing Protein 8D (LRRC8D)

By searching protein and nucleotide databases for sequences similar to LRRC8 (LRRC8A), followed by RT-PCR of peripheral blood mononuclear cell and monocyte total RNA, Kubota et al. (2004) identified LRRC8D as LRRC5. The deduced 858-amino acid protein contains 4 N-terminal transmembrane domains and 16 C-terminal leucine-rich repeats. LRRC8D shares 55% identity with LRRC8A and 95% identity with the 859-amino acid mouse ortholog. Semiquantitative PCR detected variable LRRC8D expression in all tissues examined except skeletal muscle.Kubota et al. (2004) found that expression of LRRC8D in isolated human peripheral blood mononuclear cells or monocytes was increased following exposure to phytohemagglutinin, phorbol ester, or lipopolysaccharide.