Lin-7 Homolog A (LIN7A)

Human VELI1 contains 233 amino acids, with a PDZ domain spanning residues 117 to 190.MPP7 formed a tripartite complex with DLG1 and LIN7A or LIN7C in vitro and in vivo. MPP7 dimerized with the LIN7 proteins through its L27C domain. The LIN7/MPP7 dimer then linked to DLG1 though the L27N domain of MPP7. This complex localized to epithelial adherens junctions in transfected Madin-Darby canine kidney cells. Expression of an MPP7 construct lacking either the PDZ or SH3 domain redistributed MPP7, DLG1, and LIN7 into the soluble cytoplasmic fraction. Rat Lin7a, which they called Mals1, was expressed specifically in brain. Sucrose gradient centrifugation showed enrichment of Mals proteins in rat cerebral cortex synaptosomes. Mals1 had an apparent molecular mass of 30 kD by Western blot analysis.