MACRO Domain Containing Protein 1 (MACROD1)

LRP16 encodes a 325-amino acid protein.Zhao et al. (2005) reported that ectopic expression of LRP16 stimulated proliferation of MCF7 breast cancer cells. LRP16 expression was stimulated by 17-beta-estradiol (E2), and a 2.6-kb fragment upstream of the translation start site of LRP16 contains ESR1 responsiveness. Using 5-prime deletion constructs in MCF7 and HeLa cells, Zhao et al. (2005) showed that the LRP16 proximal region of -251 to -214 bp contributed to E2-induced transactivation, via an estrogen-responsive element half-site and an SP1 binding site. Gel mobility shift and chromatin immunoprecipitation assays showed that ESR1 and SP1 bound directly to DNA and were required for E2-induced transactivation of LRP16.