MCF2 Cell Line Derived Transforming Sequence Protein (MCF2)

MCF2 is a member of a large family of GDP-GTP exchange factors that modulate the activity of small GTPases of the Rho family. Five-prime recombinations result in the loss of N-terminal codons, producing MCF2 variants with oncogenic potential.MCF2 is the designation of a transforming sequence identified using cotransfection of DNA from a human mammary carcinoma cell line.A comparison of the restriction maps of the DBL and MCF2 oncogenes, together with their chromosomal localization, indicates that they represent the same genetic locus. The DBL oncogene was initially detected as a transforming gene from a human diffuse B-cell lymphoma and was isolated as a 45-kb transforming human DNA sequence by cosmid cloning.MCF2 gene contains 25 exons and spans 85 kb.