MIS12, MIND Kinetochore Complex Component Homolog (MIS12)

The deduced 205-amino acid protein contains 2 conserved blocks in its N-terminal 100 amino acids, followed by a coiled-coil region of about 50 amino acids. Endogenous HeLa cell MIS12 had an apparent molecular mass of about 25 kD. Immunolocalization showed MIS12 colocalized at kinetochores with CENPA and CENPC, and its position during the cell cycle was that of a typical kinetochore protein. The level of MIS12 did not fluctuate during the cell cycle.Immunoprecipitation of transfected cells indicated that MIS12 could form stable complexes with C20ORF172 and DC8. Much weaker interaction was detected between MIS12 and ZWINT. Exclusion chromatography revealed 3 peaks containing MIS12 and C20ORF172, but only the middle peak, which had a mass of 669 kD, contained HP1-alpha.