MYC Associated Protein With JMJC Domain (MAPJD)

MAPJD contains a JmjC domain, 2 putative nuclear localization signals, and several potential phosphorylation sites. Northern blot analysis detected a 2.3-kb transcript expressed at variable levels in all human tissues examined and in the MCF-7 human breast cancer cell line. Immunohistochemical analysis showed that NO66 localized to different subnuclear compartments in different cell lines. In most cell lines, NO66 was enriched in the granular part of nucleoli and in distinct nucleoplasmic granules, where it colocalized with replicating chromatin. Western blot analysis of cultured human cells detected a 79-kD NO66 protein. Homologs of NO66 were detected in cell lines from diverse species, but the sizes of the proteins differed from that of human NO66, primarily due to different lengths of the N-terminal end.