Membrane Associated Ring Finger Protein 8 (MARCH8)

MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins.The deduced 291-amino acid protein has an N-terminal BKS-PHD domain, followed by 2 transmembrane domains. Both the N and C termini were predicted to be cytoplasmic. RT-PCR analysis detected MIR expression in neonatal brain and in adult lymph node, spleen, and placenta, but not in other tissues examined. It was also detected in monocytes and immature dendritic cells, but not in lymphocytes.