Microtubule Associated Monoxygenase, Calponin And LIM Domain Containing Protein 3 (MICAL3)

All members of the evolutionarily conserved MICAL family of proteins have an N-terminal monooxygenase domain containing flavin adenine dinucleotide (FAD)-binding motifs, followed by a calponin  homology domain, a LIM domain, and an ERM alpha domain-like coiled-coil motif. In situ hybridization of rat brain detected Mical3 expression in spinal cord, dorsal root ganglia, and sympathetic ganglia at embryonic days 15 and 18. The expression patterns of Mical1, Mical2, and Mical3 appeared to overlap but were distinct. RT-PCR ELISA detected intermediate expression in adult brain, lung, liver, kidney, testis, ovary, fetal liver, and most specific brain regions examined. Lower levels were detected in heart and fetal brain, and little to no expression was detected in skeletal muscle, pancreas, and spleen.