Mindbomb Homolog 1 (MIB1)

The DIP1 transcript contains a 3-prime repeat sequence. RT-PCR ELISA detected moderate to high DIP1 expression in all tissues examined, with highest levels in spinal cord, ovary, whole brain, and all specific brain regions examined.deduced 1,006-amino acid protein contains a zinc finger, 9 ankyrin repeats, 3 RING fingers, and an alpha-helical coiled-coil region between RING2 and RING3. Northern blot analysis detected a 4.0-kb transcript in all mouse tissues analyzed. HeLa cells transiently expressing mouse Dip1 or only the Dip1 RING finger motifs showed DNA fragmentation characteristic of apoptotic changes. Overexpression of Dip1 abolished the antiapoptotic effect of Dapk expression. Dip1 regulated the cellular levels of Dapk through its E3 ubiquitin ligase activity, which promoted ubiquitination of Dapk in vitro and in vivo.