NLR Family, Pyrin Domain Containing Protein 12 (NLRP12)

PYPAF7 associated with ASC (PYCARD) in a PYD-dependent manner. Immunoblot and luciferase reporter analyses showed that ASC and PYPAF7 synergistically participated in activation of NFKB and procaspase-1. PYPAF7 has a role in the transduction of proinflammatory signals leading to activation of NFKB and procaspase-1.The predicted 1,061-amino acid protein has an N-terminal PYRIN domain, a central NACHT-type nucleotide-binding site domain, and a C-terminal domain with at least 12 LRR motifs. Northern blot and RT-PCR analyses revealed expression in peripheral blood leukocytes, with little to no expression in 75 other tissues and cell lines examined. Real-time PCR detected expression predominantly in eosinophils and granulocytes, with lower expression in monocytes.