Na-K-Cl Cotransporter 1 (NKCC1)

By moving chloride into epithelial cells, the Na-K-Cl cotransporter SLC12A2 aids transcellular movement of chloride across both secretory and absorptive epithelia.The deduced 1,212-amino acid protein contains 12 transmembrane segments and shows 91% identity to mouse Nkcc1. Two sites for N-glycosylation were predicted on an extracellular loop between putative transmembrane segments 7 and 8, and a single potential phosphorylation site was present in the predicted cytoplasmic C-terminal domain. The molecular mass of the expressed protein is approximately 132 kD.In contrast to the renal-specific sodium/potassium/chloride cotransporter SLC12A1, SLC12A2 is expressed in many tissues, including the basolateral membrane of secretory epithelia.