Nipsnap Homolog 1 (NIPSNAP1)

Nipsnap1 expression in all mouse tissues examined except spleen. Immunohistochemical analysis of kidney sections detected Nipsnap1 in the inner medulla collecting ducts. Coprecipitation, protein pull-down, and functional assays showed that Nipsnap1 interacted with Trpv6 and regulated its channel activity. Electrophysiologic recordings revealed that Nipsnap1 abolished Trpv6 currents, but it did not alter the membrane localization of Trpv6.The predicted 284-amino acid protein, which they called NIPSNAP1, shares 94% sequence identity with the mouse ortholog, Nipsnap1. Northern blot analysis revealed that NIPSNAP1 is expressed ubiquitously as a 2.1-kb mRNA, with the highest level in liver. The authors determined that the NIPSNAP1 gene contains 10 exons and spans 26 kb.