Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)

DR-nm23 mRNA was preferentially expressed at early stages of myeloid differentiation of highly purified CD34(+) cells. Its constitutive expression in a myeloid precursor line, which is growth-factor dependent for both proliferation and differentiation, resulted in inhibition of granulocytic differentiation induced by granulocyte colony-stimulating factor and caused apoptotic cell death.The deduced 169-amino acid protein shares 88% identity with human NME3. Northern blot analysis of several mouse tissues detected a 0.9-kb transcript expressed at highest levels in liver and kidney, with moderate levels in heart, brain, spleen, and lung. Little to no expression was detected in other mouse tissues examined. In situ hybridization of 15-day postcoitum mouse embryos showed expression in nervous tissue and thymus.