NudC Domain Containing Protein 3 (NUDCD3)

NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of this gene product results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. The protein localizes to the Golgi apparatus during interphase, and levels of the protein increase after the G1/S transition.The deduced 361-amino acid protein has a calculated molecular mass of 41 kD. The N-terminal half contains a coiled-coil domain, and the C-terminal half contains a nuclear movement domain and a p23 (PTGES3) domain. Western blot analysis detected ubiquitous Nudcl expression in all mouse tissues tested. NUDCL colocalized with Golgi marker proteins in HeLa cells during interphase and mitosis.