PCF11 Homolog (PCF11)

De Vries et al. (2000) purified pre-mRNA cleavage factor IIm (CFIIm) of the 3-prime end processing complex from HeLa cell nuclear extracts and determined that it contains PCF11 and CLP1.The deduced protein contains 1,644 amino acids. RT-PCR ELISA detected intermediate expression in all adult and fetal tissues and all specific brain regions examined.The C-terminal domain motif of ser2-pro3-thr4-ser5 forms a beta turn that binds to a conserved groove in the C-terminal domain-interacting domain of PCF11. The ser2 phosphate group does not make direct contact with PCF11, but may be recognized indirectly because it stabilizes the beta turn with an additional hydrogen bond. Iteration of the peptide structure results in a compact beta spiral model of the C-terminal domain.