POTE Ankyrin Domain Family, Member E (POTEE)

The longest deduced protein, POTE2A, contains 712 amino acids and has a calculated molecular mass of 72.5 kD. POTE2A has 3 cysteine-rich N-terminal repeats, followed by 7 ankyrin repeats and a C-terminal helical region. The deduced 394-amino acid POTE2B protein has a calculated molecular mass of 40.2 kD. Compared with POTE2A, POTE2B has extra sequence within the ankyrin repeat region and lacks the C-terminal helical region. POTE2C and POTE2D differ only in the long 3-prime UTR. The deduced 376-amino acid POTE2C/POTE2D protein has a calculated molecular mass of 38.6 kD. Compared with POTE2A, POTE2C/POTE2D lacks the C-terminal helical region. In vitro transcription and translation of the POTE2 variants resulted in proteins with molecular masses in agreement with their calculated masses.