Patched Domain Containing Protein 3 (PTCHD3)

Fan et al. (2007) cloned 2 splice variants of mouse Ptchd3. The deduced proteins contain 410 and 906 amino acids, of which the first 409 residues are identical. Northern blot analysis of mouse tissues detected 2 transcripts in testis only. RT-PCR showed Ptchd3 expression starting around day 16 of mouse postnatal development, suggesting that Ptchd3 is expressed around the pachytene stage of spermatogenesis.Expression of Ptchd3 was not detected in mice lacking germ cells. Immunofluorescence analysis localized PTCHD3 to the midpiece of human, mouse, and rat sperm.Fan et al. (2007) stated that the PTCHD3 gene maps to human chromosome 10p12.1 and to mouse chromosome 11E2.