Phenazine Biosynthesis Like Protein Domain Containing Protein (PBLD)

By yeast 2-hybrid screening of a human liver cDNA library using the WD40 repeat region of MAWD (STRAP) as bait, Iriyama et al. (2001) obtained a full-length cDNA encoding PBLD, which they called MAWBP.The deduced protein contains 255 amino acids and is highly conserved from single-cell to higher eukaryotes. It shares 37% amino acid identity with the Arabidopsis enzyme Phzf, which catalyzes hydroxylation of phenazine-1-carboxylic acid to 2-hydroxy-phenazine-1-carboxylic acid. RT-PCR detected variable expression of MAWBP in all human tissues examined except skeletal muscle.By somatic cell hybrid and radiation hybrid analyses, Iriyama et al. (2001) mapped the PBLD gene to chromosome 10q21.1.