Phosducin Like Protein 3 (PDCL3)

VIAF overexpression did not alter the ability of Op-IAP to prevent BAX-mediated apoptosis. However, suppression of VIAF by RNA interference in human embryonic kidney cells inhibited processing of caspase-3 (CASP3) following induction of the apoptotic program by BAX. VIAF was a substrate for Op-IAP-mediated ubiquitination, suggesting that VIAF may be regulated by IAP proteins in a ubiquitination-dependent manner. Inhibitor of apoptosis proteins (IAPs) are involved in suppression of apoptosis, signal transduction, cell cycle control, and gene regulation. The deduced 239-amino acid protein has a calculated molecular mass of 28 kD. Northern blot analysis detected variable expression of a 1.2-kb transcript in all tissues examined. Epitope-tagged and endogenous VIAF showed cytoplasmic expression.