Protein Inhibitor Of Activated STAT 4 (PIAS4)

Using PIAS1 for database searches and cDNA library screening, they identified other related genes including PIASX and PIASY. PIASY encodes a deduced 510-amino acid protein. Like other members of the PIAS family, the predicted PIASY protein contains a putative zinc-binding motif and a highly acidic region. Northern blot analysis of several mouse tissues detected highest expression in testis. A similar expression pattern was observed in human tissues. Piasy was expressed in isolated mouse vascular endothelial cells and more weakly in smooth muscle cells and fibroblasts. Confocal microscopy detected PIASY in the nuclear compartment of transfected COS-1 cells, and deletion of its C-terminal half shifted PIASY to the cytoplasm.