Retinoic Acid Induced Protein 16 (RAI16)

RAI16 interacting with Tec kinase domain. TRAI16 cDNA sequence was synthesized, and then linked to pMD18-T vector. After enzyme digestion, the purified target fragment was linked to the expression vector pGEX4T-2, which was then transferred and screened. After the positive recombinants were transferred into human E.coil BL-21, the expression was induced by different concentrations of isopropyl β-D-thiogalactoside (IPTG) at different temperatures and culture time periods. The expression products were analyzed by SDS-PAGE. RAI16 cDNA was successfully cloned in pGEX4T-2 plasmid. Using 0.4 mmol/L IPTG at 30℃ for 4 h, the soluble target protein was expressed efficiently. SDS-PAGE revealed glutathione S-transferase-RAI16 fusion protein bands expressed mainly in the form of inclusion bodies.