Ribonucleoprotein, PTB Binding 1 (RAVER1)

Northern blot analysis of adult mouse tissues detected ubiquitous Raver1 expression, with highest levels in striated muscle, liver, kidney, and testis, and lowest levels in brain, lung, and spleen. Raver1 is a dual compartment protein, localized at microfilament plasma membrane attachment sites in fibroblasts, epithelial cells, and muscle, and, like polypyrimidine tract-binding protein (PTB), within perinucleolar structures that are engaged in RNA metabolism. Interspecies heterokaryon assays showed that Raver1 can translocate between the nucleus and cytoplasm. Immunofluorescence microscopy in mouse C2C12 myoblasts showed that translocation of Raver1 from the nucleus to the cytoplasm in skeletal muscle correlated with the differentiation of specific microfilament attachment sites.