Ribosomal Protein S16 (RPS16)

RPS16 encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S9P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.On Northern blots, the RPS16 cDNA hybridized to a 650-bp transcript showing 30-fold higher expression in Panc-1 cells than in CD11 cells. The RPS16 transcript was not overexpressed in the other 13 pancreatic tumor cell lines examined or in 2 breast cancer cell lines, 2 colon cancer cell lines, or normal tissues. Southern blot analysis showed a 20-fold amplification of the RPS16 gene only in the Panc-1 cell line. The deduced 146-amino acid RPS16 protein is identical to rat Rps16.