Ring Finger Protein 167 (RNF167)

RNF167 is an E3 ubiquitin ligase that interacts with TSSC5 (SLC22A18) and, together with UBCH6 (UBE2E1), facilitates TSSC5 polyubiquitylation . Immunoprecipitation analysis with UBCH5A (UBE2D1) and UBCH6 showed that RING105 had autoubiquitylation activity. Bacterial 2-hybrid screening with RING105 as bait revealed an interaction with TSSC5, which, like RING105, localized to cytoplasmic membranes. Immunoprecipitation and immunoblot analyses with truncated forms of TSSC5 demonstrated that TSSC5 was polyubiquitylated on its sixth hydrophilic loop in an accelerated manner in the presence of RING105 and UBCH6. FACS analysis showed that RING105 delayed cell-cycle transition in a RING finger domain-dependent manner.