Ring1 And YY1 Binding Protein (RYBP)

Using mouse Ring1 as bait in a yeast 2-hybrid screen, Garcia et al. (1999) cloned Rybp from a mouse embryo cDNA library. The deduced 227-amino acid protein has an N-terminal C2C2 zinc finger motif and shows blocks of sequence identity with human YAF2 ranging from about 80% in the zinc finger domains to about 45% at the C termini.Using the non-DNA-binding subunit of GABPB as bait in a yeast 2-hybrid screen, Sawa et al. (2002) cloned human RYBP, which they called YEAF1, from a HeLa cell cDNA library. Northern blot analysis detected ubiquitous expression of a 5-kb RYBP transcript, with highest expression in placenta.