Rogdi Homolog (ROGDI)

ROGDI is a novel gene which locates on human chromosome 16p13.3 and has unknown function. The length of ROGDI coding region is 864 bp which encodes 287 amino acids. CDK2 is a member of cyclin-dependent kinases (CDKs) which plays a key role in regulation of G1/S cell cycle transition and is activated during G1 and S phases. On the other hand, down-regulation of CDK2 and cyclin A was observed following silencing of ROGDI. In addition, the interaction between ROGDI and CDK2 was evidenced by using immunoprecipitation. ROGDI-enhanced cell proliferation was dependent on CDK2 activity. Flow cytometry profiles of depletion of ROGDI displayed G1/S phase arrest as well as up-reguated the expression of p53 and p21. ROGDI may be a positive regulator of cell cycle and its action is CDK2-depedent.