SEC14 Like Protein 3 (SEC14L3)

SEC14L3 shares 86% similarity with SEC14L2. All 3 proteins share residues glu185 and lys216, which are predicted to play a role in phospholipid binding and transfer activity in S. cerevisiae Sec14. RT-PCR of human tissues and cell lines detected SEC14L3 expression in all tissues examined, with lower expression in skin, blood, and liver. Immunocytochemical studies localized SEC14L3 to intracellular membranes of the cytoplasm in HeLa cells, including partial localization to the ER and Golgi, with more intense staining surrounding but not within the nucleus.The deduced 400-amino acid protein has a calculated molecular mass of 46.1 kD. SEC14L3 has a conserved CRAL/TRIO domain and shares 76% homology to SEC14L2. SEC14L3 expression in liver only by RT-PCR analysis.