SEC22 Vesicle Trafficking Protein Homolog B (SEC22B)

In S. cerevisiae, the vesicle trafficking protein complexes directing transport between the endoplasmic reticulum (ER) and Golgi appear to include Sed5, proposed to be a cis-Golgi receptor protein, and Sec22 and Bet1, potential Sed5 docking partners localized on ER-derived vesicles. The complex contained syntaxin-5, GOS28 , the rat homologs of Bet1 and Sly1, and 2 novel proteins, rat SEC22B and membrin. Other members of the complex localized to Golgi membranes, indicating that the complex recapitulates vesicle docking between distinct organelles in the ER/Golgi transport cycle. Expression of recombinant membrin and Sec22b disrupted normal trafficking, demonstrating that these proteins regulate ER-to-Golgi trafficking.