STAM Binding Protein Like Protein 1 (STAMBPL1)

The cDNA contains several repetitive elements in its 3-prime end, and the deduced 463-amino acid protein shares significant similarity with AMSH (STAMBP). RT-PCR ELISA revealed highest STAMBPL1 expression in testis and ovary, with much lower expression in whole adult brain. Among specific adult brain regions, STAMBPL1 was detected in cerebellum only.Northern blot analysis detected a 2.5-kb AMSHLP transcript in all human tissues examined, with highest expression in testis. Minor transcripts of 5.3 and 5.5 kb were expressed in spleen and weakly in thymus and heart. Western blot analysis detected a 50-kD protein in all mouse and human cell lines examined. In transfected COS-7 cells, fluorescence-tagged AMSHLP was expressed diffusely in the cytoplasm and in a punctate pattern surrounding the nuclear membrane.