Signal Peptidase Complex Subunit 1 Homolog (SPCS1)

By RT-PCR of a human fetal brain cDNA library using degenerate oligonucleotides based on canine Spcs1, Kailes and Hartmann (1996) cloned human SPCS1.The 102-amino acid protein has a predicted molecular mass of 11.8 kD. SPCS1 shows no sequence similarity to any of the other signal peptidase complex subunits.SPCS1 contains 2 predicted transmembrane domains, and immunoblot analysis of protease-digested canine microsomes showed that both the N- and C-terminal domains of Spcs1 and Spcs2 are cytoplasmic.The International Radiation Hybrid Mapping Consortium mapped the SPCS1 gene to chromosome 3 .