Small Nuclear Ribonucleoprotein Polypeptide G (SNRPG)

One common protein, G (SNRPG), migrates as a doublet of approximately 9 kD by high-TEMED SDS-PAGE. By performing RACE on a HeLa cell cDNA library using primers based on the partial protein sequence of SNRPG, Hermann et al. (1995) cloned SNRPG cDNAs.The predicted 76-amino acid protein contains 34% hydrophobic residues, most of which are located in the C-terminal half. The authors found that G protein translated in vitro from a single SNRPG mRNA also migrated as a doublet by high-TEMED SDS-PAGE. They suggested that the 2 bands represent conformational isomers of the same protein. Northern blot analysis revealed that the SNRPG gene is expressed as an approximately 0.5-kb mRNA in HeLa cells.