Sodium Dependent Dicarboxylate Cotransporter 3 (NADC3)

The SLC13A3 cDNA encodes a deduced 602-amino acid protein with 12 transmembrane domains and a molecular mass of 66.9 kD. The protein also has 8 potential protein kinase C-dependent phosphorylation sites, 1 potential cAMP/cGMP-dependent protein kinase phosphorylation site, and 3 putative N-linked glycosylation sites. SLC13A3 also shares 45% sequence identity with the human low-affinity dicarboxylate transporters. The human and rat SLC13A3 proteins share 85% sequence identity, indicating that the protein is highly conserved across the species. Northern blot analysis detected expression of an approximately 3.6-kb transcript at highest levels in kidney, but also in placenta, brain, liver, and pancreas; no expression was detected in heart, lung, or skeletal muscle.