Solute Carrier Family 10, Member 6 (SLC10A6)

The deduced 377-amino acid protein has a calculated molecular mass of 41.2 kD. SOAT contains 7 to 9 predicted transmembrane domains, 3 N-glycosylation sites, and numerous potential serine and threonine phosphorylation sites. Real-time quantitative PCR showed that SOAT expression was very high in testis, high in placenta and pancreas, moderate in heart, lung, and mammary gland, and low in brain, colon, kidney, liver, ovary, small intestine, spleen, thymus, and adrenal gland. Characterization of the SOAT protein expressed in HEK293 cells revealed an extracellular N terminus and an intracellular C terminus, suggesting that SOAT has either 7 or 9 transmembrane domains, but not 8. Glycosylated and deglycosylated SOAT proteins had apparent molecular masses of 45 and 41 kD by SDS-PAGE, respectively.