Solute Carrier Family 4, Member 9 (SLC4A9)

SLC4A9 contains 945 amino acids and has a calculated molecular mass of 104 kD. AE4 has 14 transmembrane domains and intracellular N and C termini. It contains an N-terminal leucine zipper motif, multiple intracellular phosphorylation sites, and 2 extracellular N-glycosylation sites. It shares 84% amino acid identity with rabbit Ae4, a sodium-independent Cl-/HCO3- exchanger located on the apical membrane of beta-intercalated kidney cells. SDS-PAGE following cell-free translation revealed AE4 at an apparent molecular mass of 100 kD. AE4 did not appear to be N-glycosylated. Northern blot analysis detected expression in kidney only.The major splice variant encodes a 990-amino acid protein that shares 49% and 48% identity with SLC4A4 and SLC4A7, respectively.