Spermatogenesis And Centriole Associated Protein 1 Like Protein (SPATC1L)

Using a high-throughput growth inhibition assay to identify genes conferring sensitivity or resistance to DNA alkylation, Fry et al. (2008) identified C21ORF56. Database analysis revealed 340- and 186-amino acid isoforms of C21ORF56 that used different start methionines. C21ORF56 orthologs were detected in chimp, mouse, rat, dog, cow, and opossum.By examining gene expression profiles, Fry et al. (2008) showed that elevated C21ORF56 expression was associated with reduced sensitivity to MNNG, a DNA alkylating agent. Knockdown of C21ORF56 increased the sensitivity of a lymphoblast cell line to MNNG-induced cell killing.By genomic sequence analysis, Hattori et al. (2000) mapped the C21ORF56 gene to chromosome 21q22.3.