Splicing Factor 1 (SF1)

The predicted 623-amino acid protein contains a nuclear transport domain, a metal-binding or zinc finger motif, and glutamine- and proline-rich regions. It shows some sequence similarity to WT1 and EGR2. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary.All of the ZNF162 isoforms contain a KH domain, a sequence motif present in proteins playing a major role in regulating cellular RNA metabolism. The gene contains 14 exons and spans approximately 15 kb. Analysis of the ZNF162 genomic cDNA sequences predicted the use of duplicated 5-prime and 3-prime splice sites as well as exon skipping and intron inclusion to generate 6 ZNF162 mRNAs by alternative splicing events.