Sprouty Related, EVH1 Domain Containing Protein 1 (SPRED1)

SPRED1 is a member of the Sprouty family of proteins and is phosphorylated by tyrosine kinase in response to several growth factors. The encoded protein can act as a homodimer or as a heterodimer with SPRED2 to regulate activation of the MAP kinase cascade. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). Overexpression of Spred1 inhibited NGF-induced neurite differentiation in rat PC12 cells, and both the EVH1 and SPRY domains were essential for the suppression.mouse Spred1 coprecipitated with and was phosphorylated by a constitutively activated Kit mutant. The KBD was required for Kit binding, but suppression of growth factor-induced MAP kinase required the SPRY domain.