Syntaxin 12 (STX12)

Rat STX12 shares 53% sequence identity with STX7. Northern blot analysis detected expression of a 2.9-kb transcript in rat brain, with very little expression in other tissues. Western blot analysis detected a 33-kD band in brain, a faint signal in pancreas, and barely detectable signals in other tissues. Cell fractionation studies of rat brain showed that STX12 is an integral membrane protein, and immunolocalization studies localized STX12 to the cell body of cultured rat hippocampal neurons. Northern blot analysis detected highest expression in brain, lung, and kidney. Immunolocalization studies showed compact perinuclear staining, and experimental manipulation revealed specific localization of STX12 to the endosomal compartment.