Syntaxin 5 (STX5)

In eukaryotic cells, vesicle docking is thought to be regulated in part by the specific interactions of a vesicle-associated membrane protein (VAMP), or synaptobrevin, with the presynaptic plasma membrane proteins syntaxin and SNAP25.By PCR using oligonucleotides based on the sequence of rat syntaxin-5, Ravichandran and Roche (1997) isolated a partial cDNA encoding human syntaxin-5. They used the partial cDNA to clone a full-length human syntaxin-5 cDNA from an EBV-transformed lymphocyte cell cDNA library. The predicted 301-amino acid human protein is 96% identical to rat syntaxin-5. In vitro, human syntaxin-5 bound efficiently to rat VAMP2 but not to human SNAP25.