T-Cell Activation Kelch Repeat Protein (TAKRP)

The gene of TA-KRP was cloned from A549 cell, and the protein of TA-KRP was harvested in prokaryotic expression system. The reaction of nsp9 to TA-KRP in vitro will be proved by pull-down experiment. Laser scanning confocal microscropy detected distributed cytoplasmic fluorescence foci of nsp9 with IgG-anti-nsp9(FITC),and also demonstrate colocalization of nsp9 and TA-KRP in cytoplasmic complex. N-terminal sequenceing of peptide showed the other potential receptor protein of nsp9 was Beckwith-Wiedemann syndrome chromosome region 2 associated zinc-finger protein 2(BAZ2,ZNF215). The reaction of nsp9 to BAZ2 in vitro was proved by pull-down experiment. And also nsp9 and BAZ2 colocalized with each other in cytoplasmic complex. The reaction of nsp9 to TA-KRP and to BAZ2 in vivo will be proved by cosedimentation assays.