TPA Induced Trans Membrane Protein (TTMP)

By microarray gene expression profiling using RNA from pancreatic cells treated with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), followed by 5-prime and 3-prime RACE of human colon cDNA, Chan et al. (2005) cloned C3ORF52, which they called TTMP. The deduced 217-amino acid protein has a calculated molecular mass of 24.3 kD and contains a central transmembrane domain predicted to span the endoplasmic reticulum membrane. C3ORF52 shares 68% amino acid identity with the mouse homolog. Chan et al. (2005) demonstrated early onset of C3ORF52 induction with a 3.4-fold increase at 2 hours after administration and maximal induction at 6 hours in pancreatic cells and at 24 hours in HeLa cells. Actinomycin D treatment showed that TPA induction of C3ORF52 occurred at the transcriptional level.