Tectonin Beta Propeller Repeat Containing Protein 1 (TECPR1)

A previously uncharacterized tectonin b-propeller repeat protein, TECPR1, associated reciprocally with ATG12, ATG3 and ATG5, indicating its involvement in the ATG8 lipidation pathway. The WD40-repeat protein ATG16L1 and TECPR1 also associated with theCCT chaperonin complex implicated in the folding of WD40 proteins10. TECPR1 also associated with FLJ12716, TTC15 and components of the TRAPP vesicle-tethering complex, including TRS85, as well as TRAPPC2L,TRAPPC3, TRAPPC4 and TRAPPC5 with subthreshold WDN-scores. In contrast, depletion of TECPR1 and its associated protein TTC15 led to an increase in autophagosome number. Interestingly, depletion of TTC15 also resulted in a defect in flux, whereas depletion of TECPR1 did not, indicating that these proteins act at distinct steps in the process.