TraB Domain Containing Protein (TRABD)

Chromosome 22 contains over 500 genes and about 49 million bases. Being the second smallest human chromosome, 22 contains a surprising variety of interesting genes. Phelan-McDermid syndrome, Neurofibromatosis type 2 and autism are associated with chromosome 22. A schizophrenia susceptibility locus has been identified on chromosome 22 and studies show that 22q11 deletion symptoms include a high incidence of schizophrenia. An actin binding assay system was used to examine the ability of GST-TrABD, a GST fusion protein comprising trabeculin’s a-actinin-like actin binding domains, to bind to F-actin. The GST-TrABD fusion protein was generated as described above. a-Actinin and BSA were used as positive and negative controls, respectively, and the assay was carried out essentially as described.