Trafficking Protein Particle Complex 3 (TRAPPC3)

TRAPPC3 is a component of the TRAPP complex, which is involved in tethering of transport vesicles to the cis-Golgi membrane.TRAPPC3 contains 180 amino acids. Bet3 expression in all mouse tissues examined. Western blot analysis of fractionated rat liver showed that most Bet3 was present in the cytosolic fraction, with a lesser amount in the Golgi-enriched membrane fraction.Cytosol depleted of Bet3 was also defective in this transport and could be rescued by recombinant Bet3. Bet3 acted after coat protein II (COPII) but before Rab1, alpha-Snap (NAPA), and the EGTA-sensitive stage during ER-Golgi transport.Both wildtype yeast Bet3 and mutant yeast Bet3 lacking palmitoylation rescued cell viability in Bet3-deleted yeast, suggesting that palmitoylation is not required for cell viability.