Transcription Factor Like Nuclear Regulator (TFNR)

The TFNR protein is highly hydrophilic. It contains a 55-amino acid motif that is repeated as 6 highly conserved and 3 less well-conserved copies encoded by exon 17. It also contains a bipartite nuclear localization signal, a DNA-ligase A1 ATP-dependent binding site, and a second bipartite nuclear localization signal in the C terminus. Sequence comparison with the first 11 exons of mouse Tfnr, encoding 545 amino acids, indicated 77% identity to the human TFNR protein. A stretch of over 300 amino acids of human TFNR showed 29% identity with the yeast TFC5 protein, a component of the TFIIIB transcription initiation complex. Northern blot analysis detected weak expression of 9.5-, 3.1-, and 1.6-kb transcripts in all tissues examined, and a 1.9-kb transcript expressed only in brain.