Transcription Termination Factor, RNA Polymerase II (TTF2)

TTF2 has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.The deduced 990-amino acid TTF2 protein shares 43% amino acid sequence identity with Drosophila factor 2. These proteins contain 7 motifs found in helicase superfamilies 1 and 2; 7 additional motifs shared only by SWI2/SNF2 family members; and 3 conserved motifs and a nuclear localization signal that are not conserved among the SWI2/SNF2 family. Similar to Drosophila factor 2, recombinant human TTF2 displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity.