Translocation Associated Membrane Protein 2 (TRAM2)

TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane.TRAM2 shares 51.6% identity with human RA-regulated nuclear matrix associated protein, RAMP. Northern blot analysis detected moderate expression in all tissues examined except brain.TRAM2 also coprecipitated with collagen. Deletion of the C terminus of TRAM2, and pharmacologic inhibitors of SERCA2b, inhibited type I collagen synthesis. In addition, depletion of ER Ca(2+) inhibited the folding of triple helical collagen and increased its intracellular degradation.